P-167 Bacterial contamination of embryo culture medium in conventional in vitro fertilisation versus ICSI

Abstract Study question Our aim is presenting a clinical case of pregnancy using intracytoplasmatic sperm injection with bacterial contamination embryo culture after failed conventional in vitro fertilisation. Summary answer Intracytoplasmatic (ICSI) can be an effective and safe method to prevent infection microbial semen. What known already Contamination rare, but detrimental. The most common sources are semen follicular fluid. We currently have no standard protocols for monitoring biological fluids. Semen often contains bacteria even if hygiene rules collection followed. Most studies found significant difference the effectiveness fertilisation treatment presence or absence bacteriospermia. This explained by preparation techniques, antibiotic content media use ICSI. centres advise against contaminated transfers, some achieved removing zona pellucida design, size, duration report two consecutive - ICSI cycles at Division Assisted Reproduction, Department Obstetrics Gynecology, Semmelweis University, Budapest 2022. Participants/materials, setting, methods First we performed couple secondary infertility because oligoasthenozoospermia. For second time suggested due growth observed medium. Microbiological analyses were carried out. video-recorded cytoplasmic one oocyte following time-lapse microscopy. Main results role chance After proliferation rods control medium was sterile. infected contained Escherichia coli Staphylococcus hominis. oocytes did not fertilize degenerated. husband asymptomatic alteration physical examination therefore he treated antibiotics. In next cycle 2 14 fertilized normally developed into high-quality blastocysts. One transferred resulting live birth cryopreserved. video-documented fertilised others. Infected removed from its showed Cutibacterium acnes. Fungal sexually transmitted diseases negative. only coli. cervix resistant gentamicin Limitations, reasons caution had 30 years conclude routine infections fluids present. Wider implications findings offered patients help reducing risk culture. disadvantages excessive treatments. New guidelines needed how handle media. Trial registration number applicable